The pair came together for the customary face-off after they had weighed in but Tyson took the opportunity to lash out as he slapped the YouTuber.
Jake Paul sent a final warning shot to Mike Tyson during the final face-off for their blockbuster bout on Friday night.
Paul and Tyson hit the scales and saw each other for the final time, less than 24 hours before they meet in the ring at the AT&T Stadium in Texas.
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The fight is already surrounded with controversy because of the age difference.
The pair came together for the customary face-off after they had weighed in but Tyson took the opportunity to lash out as he slapped the YouTuber.
In response to Paul, 27, entering his personal space, Tyson unleashed an open-handed slap that connected flush on Paul's face. The two were quickly separated by security as Paul played to the crowd, mocked Tyson and laughed at him.
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The fight was due to take place in July, but just two months after the bout was announced, the two men were forced to postpone proceedings.
Tyson was restrained and gave little comment before leaving the stage. That gave Paul an opportunity on the microphone to send his final message.
Speaking to Ariel Helwani on stage, Paul said: "Mike Tyson, that was a cute slap buddy but tomorrow you’re getting knocked the f*** out. ‘I’m f*****g him up, I’m f*****g him up! He hits like a b****. It’s personal now, it’s personal now!